Mechanism of molecular interactions for tRNA^Val recognition by valyl-tRNA synthetase

Change in the orientation of the ValRS coiled-coil domain. (A) Structural comparison between the tRNA^Val-bound ValRS and the ligand-free ValRS. The Rossmann-fold domains are superposed. The coiled-coil domains of the tRNA^Val-bound ValRS and the ligand-free ValRS are yellow and green, respectively. The AS-binding domains of the tRNA^Val-bound ValRS and the ligand-free ValRS are light yellow and light green, respectively. This figure suggests the dynamic movement of the coiled-coil domain. (B) A solvent-flattened and NCS-averaged F_o electron density map of ValRS, contoured at the 1.0σ level.