The impact of read depth and read length on RNA-seq splicing analysis

TABLE 1.

Mean fraction and mean absolute number of junction-spanning reads among uniquely mapping reads over all replicates from U2AF2 KD experiment

Read length (nt) Percentage and absolute number of junction-spanning reads (mean of two replicates)
36 20.9 (3,230,342)
50 29.6 (4,753,796)
75 46.1 (7,601,543)
100 62.3 (10,182,618)

This Article

  1. Published in Advance April 22, 2026, doi: 10.1261/rna.080915.125 RNA 32: 993-1004

Current Issue

  1. July 2026, 32 (7)
  1. Current Issue
  1. Alert me to new issues of RNA