Canonical microRNA loss drives tumor development, implicating therapeutic efficacy of enoxacin in angiosarcoma

Dgcr8 conditional deletion with aP2-Cre. (A) Graphic of Dgcr8^cKO and aP2-Cre alleles and breeding scheme to generate aP2-Cre;Dgcr8^cKO animals. Mouse illustration from the NIAID NIH BIOART Source (bioart.niaid.nih.gov/bioart/279). (B) Map of primers for genotyping the Flox, wild-type, and Cre excised alleles by genomic PCR. (C) Genomic PCR of DNA from controls, quadriceps muscle, or brown adipose tissue of wild-type (WT) or aP2-Cre;Dgcr8^Fl/Fl (cKO) animals. (D) Normal inguinal white adipose tissue H&E from a wild-type control (Cre−, Dgcr8^Fl/Fl) or Cre+, aP2-Cre;Dgcr8^Fl/Fl (cKO) animal; scale bar, 100 µm.