RK-33 suppresses OC43 replication independently of DDX3X. (A) MRC-5 cells were fixed after siRNA knockdown and OC43 infection as in Figure 1, and stained for OC43 genomic RNA by smFISH and DDX3X by IF. Representative images from four independent experiments are displayed. (B) Cells in A were segmented and within infected cells (OC43+); mean vRNA intensity was quantified on a single cell level. N > 45 cells/condition in each biological replicate from four independent experiments were analyzed. Representative analysis of replicate data is displayed. (C) The mean intensity of vRNA in OC43+ cells in B was normalized to Scramble knockdown levels across four independent experiments. (D) The mean intensity of vRNA in OC43+ cells in B was normalized to DMSO treatment across four independent replicates. Statistical significance: (ns) not significant, (*) P ≤ 0.05, (**) P ≤ 0.01, (***) P ≤ 0.001, (****) P ≤ 0.0001. Tests used: one- and two-sample t-test (C,D). Error bars represent mean and quartiles (B) or mean ± SEM (C,D).
