Effects of limited statistical power on detecting correlation between site methylation frequency and evolutionary selection. (A,B) Detection frequency of significant Spearman correlations between methylation groups (>5% methylation) and DRACH conservation under different experimental conditions: (B) with varying proportions of sites having inaccurate methylation measurements and (C) with different sample sizes. Analysis used correlation thresholds of 0.5 and 0.8, with each condition resampled 1000 times to estimate detection probability. (C) Evolutionary conservation of the central nucleotide in DRACH sites binned by methylation frequency, compared to matched control (GDBYC) sites. For DRACH sites, this represents the central adenosine conservation; for control sites, whatever central nucleotide is present at the site in humans is used as the reference.
