High-stoichiometry m⁶A sites are evolutionarily conserved

Evolutionary conservation of methylation is distinguishable from proximity to splice junction effect. (A) Distance distributions from methylated (m⁶A⁺) and unmethylated (m⁶A⁻) DRACH sites to their nearest exon junction complex (EJC). (B) Median consensus frequency score (CFS) of matched control (GDBYC) sites grouped by distance to the nearest EJC. (C,D) Conservation heat maps showing CFS for (C) DRACH sites and (D) matched control sites grouped by methylation frequency and EJC proximity. Color intensity represents conservation strength, with darker red indicating higher conservation. Numbers within cells show the count of sites per group.