3′ End-Seq reveals examples of premature termination in A. fumigatus. (A) Read alignment of AFUB_001550 encoding ortholog of S. cerevisiae ENV9; the y-axis indicates total read counts. Arrowheads indicate the positions of detected high-confidence 5′/3′ end sites. Amplified regions in B are indicated by the red lines in the gene model. The location of the intronic stop codon is indicated by a red arrow. (B) Relative expression level of exon 1 and exon 4 of AFUB_001550 measured by qPCR. Data from three biological replicates. (C) Schematic depiction of the predicted protein domain of AFUB_001550 using data from InterPro; the location of the observed internal stop site is indicated. (D) Read alignment of the previously uncharacterized AFUB_069590 gene; the y-axis indicates total read counts. Arrowheads indicate the positions of detected high-confidence 5′/3′ end sites. Amplified regions in E are indicated by the red line in the gene model. The location of the intronic stop codon is indicated by a red arrow. (E) Relative expression level of 5′ UTR and exon 5 of AFUB_069590 measured by qPCR. Data from three biological replicates. (F) Schematic depiction of the predicted protein domain of AFUB_069590 using data from InterPro; the approximate location of the observed internal stop site is indicated. Read tracks were smoothened to improve viewability, and this infrequently masks some small introns.
