A. fumigatus exhibits 5′ UTRs of typical length, but relatively longer 3′ UTRs than other fungi. (A) Histograms (bin size equals 5) of UTR length distribution based on the observed high-confidence 5′ and 3′ poly(A)-enriched RNA ends. (B) Comparison of average UTR lengths with other eukaryotes (Graber et al. 1999; Misra et al. 2002; Chen et al. 2011; Srivastava et al. 2018; Wallace et al. 2020; Hong and Jeong 2023). (C) Transcriptomic snapshot of AFUB_035670 together with the measured 5′ and 3′ end sites; the 5′ UTR of AFUB_035670 was determined to be 2150 nt long compared to the prior prediction of 703 nt in Af293. Arrowheads indicate the positions of detected high-confidence 5′/3′ end sites. Read tracks were smoothened to improve viewability, and this infrequently masks some small introns.
