Comprehensive mapping of the 5′ and 3′ untranslated regions of Aspergillus fumigatus reveals diverse mechanisms of mRNA processing including premature transcription termination

Multiple genes have transcripts with observed alternative 5′ and 3′ RNA end sites. (A) Read alignment of AFUB_097740 gene encoding a protein of unknown function, (B) AFUB_012320 encoding a putative nitrate transporter, (C) AFUB_001520 encoding 60S ribosomal protein L31e, and (D) AFUB_000040 encoding a protein with predicted RNA polymerase II transcription factor activity. Read alignment of several genes; the y-axis indicates total read counts. Arrowheads indicate the positions of detected high-confidence 5′/3′ end sites. Read tracks were smoothened to improve viewability, and this infrequently masks some small introns.