Novel trinucleotide mRNA capping reagents: improved synthetic route and efficient cotranscriptional incorporation in mRNA

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FIGURE 2.
FIGURE 2.

Structure of “d(CCCT)” DNA template, T7 promoter, and alternative forms of mRNA that could potentially form on this template with or without TCPs or ARCA. Entries 7 and 9 show the 7mGpppGmpG and 7mGpppAmpG structures in reverse orientation; however, formation of respective reverse TCP-mRNAs was not detected (see below). Positions of transcription start site and DNAzyme cleavage site are shown with arrows.

This Article

  1. Published in Advance November 19, 2025, doi: 10.1261/rna.080634.125 RNA 32: 195-214

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