miRNA targets with CIMs exhibit stronger regulatory effects. (A) The ratio of mRNA levels in the alg-1 mutant over those in wild type of all miRNA targeting sites between those without CIMs and those with deletion. The statistical significance (P-value) of the difference in fold change was calculated by the Mann–Whitney U test and the permutation test. (B) The ratio of mRNA levels in the alg-1 mutant over those in wild type of miRNA targeting sites with poor targeting scores (miRanda score <100) between those without CIMs and those with deletion. The statistical significance (P-value) of the difference in fold change was calculated by the Mann–Whitney U test and the permutation test. (C–F) The ratio of mRNA levels in the alg-1 mutant over those in wild type of miRNA targeting sites with high abundance reads (C), low abundance reads (D), both poor targeting scores and high abundance reads (E), and both poor targeting scores and low abundance reads (F) between those without CIMs and those with deletion. The statistical significance (P-value) of the difference in fold change was calculated by the Mann–Whitney U test and the permutation test.
