piRNA targets with CIMs exhibit stronger regulatory effects. (A) Distribution of piRNA targeting score (pirScan) and miRNA targeting score (miRanda) from the hybrids of PRG-1 piRNA CLASH data (left) and ALG-1 miRNA iCLIP data (right), respectively. (B) Comparison of piRNA targeting scores (left) or miRNA targeting scores (right) between those target sites without mutation and those with deletion. The statistical significance (P-value) of the difference in fold change was calculated by the Mann–Whitney U test and the permutation test. (C) Comparison of piRNA targeting scores (left) or miRNA targeting scores (right) of target sites with low abundant reads between those without mutation and those with deletion. The statistical significance (P-value) of the difference in fold change was calculated by the Mann–Whitney U test and the permutation test. (D) Comparison of local WAGO-1 22G-RNAs levels of piRNA targeting sites between those without mutation and those with deletion. The 22G-RNA levels mapped within the 100 nt window centered at the piRNA targeting site were calculated. The statistical significance (P-value) of the difference in fold change was calculated by the Mann–Whitney U test and the permutation test. (E–G) Comparison of local WAGO-1 22G-RNAs levels of piRNA targeting sites with poor targeting scores (sites with pirScan < −15) (E), low abundant reads (F), or both (G) between those without mutation and those with deletion. The 22G-RNA levels that mapped within the 100 nt window centered at the piRNA targeting site were calculated. The statistical significance (P-value) of the difference in fold change was calculated by the Mann–Whitney U test and the permutation test. (H) The ratio of local WAGO-1 22G-RNAs levels of piRNA targeting sites in the prg-1 mutant over those in wild type of all piRNA targeting sites between those without mutation and those with deletion. The statistical significance (P-value) of the difference in fold change was calculated by the Mann–Whitney U test and the permutation test. (I–K) The ratio of local WAGO-1 22G-RNAs levels of piRNA targeting sites in the prg-1 mutant over those in wild type of piRNA targeting sites with poor targeting scores (miRanda score < 100) (I), low abundant reads (J), or both (G) between those without CIMs and those with deletion. The statistical significance (P-value) of the difference in fold change was calculated by the Mann–Whitney U test and the permutation test.
