TABLE 1.
RNA aptamers used in RNP purifications
| RNA aptamer | Ligand | Ligand affinity (KD) | Chromatography resin coupling | Purified RNP | Reference |
|---|---|---|---|---|---|
| MS2 aptamer | MS2 phage coat protein fusions | 7 nM | Maltose binding protein + dextrin Sepharose resin | Ribosome precursors, RNA regulators | Said et al. 2009; Chaker-Margot et al. 2015; Zhang et al. 2016 |
| PP7 aptamer | PP7 coat protein | 0.7 nM | TAP-tagged protein + Igg resin | SNORD27 C/D box snoRNP, 7SK RNP, telomerase | Hogg and Collins 2007; Falaleeva et al. 2016; Roake et al. 2019 |
| S1 aptamer | Streptavidin | 70 nM | Streptavidin agarose resin | Poliovirus RNA, snR30 H/ACA snoRNP | Lemay et al. 2011; Leppek and Stoecklin 2014; Flather et al. 2016 |
| S1m aptamer | Streptavidin | 29 nM | Streptavidin agarose resin | Poliovirus RNA, AU-rich element of tumor necrosis factor | Leppek and Stoecklin 2014; Flather et al. 2016 |
| Csy4 binding hairpin | Csy4* protein | 50 pM | Biotinylated Csy4* protein-streptavidin resin | Identification of microRNA binding proteins | Lee et al. 2013 |
| Sephadex D8 aptamer | Sephadex (dextran) | N/D | Sephadex G100 resin | Poliovirus RNA, isolation of test RNA for proof of concept | Srisawat et al. 2001; Flather et al. 2016 |
| Sephadex D8m aptamer | Sephadex (dextran) | N/D | Sephadex G100 resin | Poliovirus RNA | Flather et al. 2016 |
| Tobramycin aptamer (J6f1) | Tobramycin antibiotic | 5.15 nM | Tobramycin-activated ester resin | Spliceosomes | Hartmuth et al. 2002 |
| Tobramycin aptamer (J6) | Tobramycin antibiotic | 0.77 nM | Tobramycin-activated ester resin | SNORD27 C/D box snoRNP, 7SK RNP, telomerase | Hogg and Collins 2007; Falaleeva et al. 2016; Roake et al. 2019 |
| Mango II aptamer | Thiazole orange (TO1) | 0.7 nM | Desthiobiotin-derivatized TO1-streptavidin resin | U1 snoRNP | Panchapakesan et al. 2017 |
