FIGURE 3.
Principle of rG4 imaging techniques. (A) Fluorescent small molecules that light up upon binding to rG4 structures. (B) Immunofluorescent staining of rG4 structure using immuno-tag G4 binding ligand. (C) Selective rG4 imaging using an antisense conjugated rG4 fluorescent ligand, or proximity-induced rolling circle amplification (RCA) triggered by click reaction between antisense and rG4-binding ligand of close proximity. (D) Fluorogenic bifunctional aptamer (FLAP) by connecting rG4 targeting aptamer and fluorogenic aptamer to image rG4 structure in the presence of its corresponding fluorescent ligand.
