Determining tDR and rsRNA identity in murine spermatocytes. (A) Representative overlay metaplots of global normalized coverage for abundant tRNA-derived reads obtained from spermatocyte RNA after using three different cDNA library preparation protocols. The fraction of reads among all tRNA-derived reads is plotted against nucleotide position (1–75) in mature tRNAs containing a 3′ terminal CCA. (B) Representative overlay metaplot of global normalized coverage for 18S rRNA-derived reads obtained from (pooled) spermatocyte RNA samples after using three different cDNA library preparation protocols. The fraction of reads among all rRNA-derived reads is plotted against nucleotide position in mature 18S rRNAs. (C) Representative overlay metaplot of global normalized coverage for 28S rRNA-derived reads obtained from spermatocyte RNA samples as in (B). The fraction of reads among all rRNA-derived reads is plotted against nucleotide position in mature 28S rRNAs.
