The effects of different library preparations on the read distribution of tDRs and rsRNAs. (A) Boxplot summarizing the total RNA content (femtograms, fg) in single murine spermatocytes from the epididymis of mice (n=14). (B) Cartoon summarizing three different approaches for preparing cDNA from extracted total spermatocyte RNA as input for next-generation small RNA sequencing. (C) Statistics of trimmed read alignment (according to cDNA library preparation protocol) to the mouse rRNA/tRNA reference sequence. Aligned reads represent uniquely mapped (blue) and multimapping reads (in red). (D) Pie charts showing the relative distribution of reads representing the most abundant RNA species in all sequenced libraries (MSC01–09).
