UPF2L complexes and RNA remodeling in the presence of UPF3B or UPF1. (A) EMSAs using 250 nM HEX_hp-RNA in all lanes. As control, HEX_hp-RNA was incubated with 10 µM UPF2L alone or 4 µM UPF3B alone. A premix of 10 µM UPF2L with HEX_hp-RNA was supplemented with increasing concentrations of UPF3B. (B) EMSAs using HEX_hp-RNA (250 nM) incubated with 10 µM UPF1 alone or 10 µM UPF2L alone. A premix of HEX_hp-RNA with 10 µM UPF2L was supplemented with increasing concentrations of UPF1. (C) EMSAs using 250 nM HEX_hp-RNA incubated with 10 µM UPF1 alone, 10 µM UPF2L alone, or 1.6 µM UPF3B alone. A premix of HEX_hp-RNA with 10 µM UPF2L and 4 µM UPF3B was supplemented with increasing concentrations of UPF1. (A–C) Remodeled RNA bands appearing in the presence of UPF2L or MIF4G domain-3 (control) are highlighted in red; RNA–protein complexes comprising UPF2L are highlighted in blue.
