Unraveling unbreakable hairpins: characterizing RNA secondary structures that are persistent after dinucleotide shuffling

(A) A histogram showing the distribution of the number of purine/pyrimidine split dinucleotides for both unbreakable and control hairpins sequences. (B) A histogram comparing the count of distinct shuffling-generatable hairpin sequences for unbreakable and control hairpin sequences. (C) Scatterplot comparing the number of distinct dinucleotide-shuffled sequences and the number of purine/pyrimidine split dinucleotides for unbreakable and control hairpins. The unbreakable hairpins have fewer, typically one, purine/pyrimidine split dinucleotides and fewer distinct dinucleotide generatable sequences.