Constructing an active chimeric pRNA ring with a stoichiometry of six and identifying 12 domains of the pRNA ring binding to the 12-subunit channel of phi29 DNA-packaging motor

The structure of the Phi29 RNA–connector complex. (A) The pRNA hexamer based on the published crystal structure. Each monomer is a different color. (B) A top-down view of the computationally derived gp10 structure, which is made of 12 subunits. (C) Side and bottom views of the connector–pRNA complex. (Green) Connector; (yellow/red) pRNA. (D) The complex showing the relative position of the N-terminal Arg–Lys–Arg (RKR) motif (blue) and the two gp10-binding regions (red and yellow) of pRNA for interaction with the RKR motif.