Constructing an active chimeric pRNA ring with a stoichiometry of six and identifying 12 domains of the pRNA ring binding to the 12-subunit channel of phi29 DNA-packaging motor

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 6.
FIGURE 6.

DNA-packaging assay of trimeric pRNA. Assay for the DNA-packaging activity of the elongated Ab′M2/Bc′M2/Ca′M2 pRNA. (A) Gel result. (Positive control) Aa′ pRNA. (Negative control) Same as positive control but with no ATP. 1% agarose gel in 1× TAE. (B) Quantification of the gel, normalized to the positive control.

This Article

  1. Published in Advance April 1, 2025, doi: 10.1261/rna.080383.125 RNA 31: 836-849

Current Issue

  1. July 2026, 32 (7)
  1. Current Issue
  1. Alert me to new issues of RNA