Constructing an active chimeric pRNA ring with a stoichiometry of six and identifying 12 domains of the pRNA ring binding to the 12-subunit channel of phi29 DNA-packaging motor

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FIGURE 5.
FIGURE 5.

Evaluation of the biological function of the dimeric pRNA. (A) Assay for the DNA-packaging activity of the elongated Ab′M2/Ba′M2 pRNA. Positive control (+c) is with Aa′ pRNA. Negative control is Aa′ pRNA but without ATP. 1% agarose gel in 1× TAE. (B) Quantification of the gel, normalized to the positive control. (C) Plaque-forming unit assay of the elongated Ab′M2/Ba′M2 pRNA.

This Article

  1. Published in Advance April 1, 2025, doi: 10.1261/rna.080383.125 RNA 31: 836-849

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