Constructing an active chimeric pRNA ring with a stoichiometry of six and identifying 12 domains of the pRNA ring binding to the 12-subunit channel of phi29 DNA-packaging motor

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 4.
FIGURE 4.

Assay for trimerization by hand-in-hand interaction of Ab′M2/Be′M2/Ea′M2 pRNA designed via complementary modifications (8% native PAGE in 1× TBM; [L] DNA ladder).

This Article

  1. RNA 31: 836-849