RNA-binding protein Miso/CG44249 is crucial for minor splicing during oogenesis in Drosophila

Reduced protein levels of MIGs in miso mutant ovaries. (A) Protein levels of MIGs in ovaries, quantified by TMT mass spectrometry. Mean ± SD (n = 4 for miso^+/+, miso^null/+, and miso^null/null + miso-EGFP; 5 for miso^null/null). P-value < 0.001 (Student's t-test, unpaired, two-tailed) is indicated by *. Results for Lsm12a, Sf3a1, and Stas are shown as representative. The results for the other MIGs and Gapdh2 are shown in Supplemental Figure S9. (B) Western blot of ovary lysates for Lsm12a.