RNA-binding protein Miso/CG44249 is crucial for minor splicing during oogenesis in Drosophila

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FIGURE 5.
FIGURE 5.

Miso associates with U11 and U12 snRNAs in ovaries. (A) Volcano plots from RIP-seq of anti-FLAG immunoprecipitates from Mat67 > UASp-HHF-Miso ovary lysates compared to IgG controls. Mean values from three biological replicates are shown. (B) RT-qPCR quantification of U1, U6, U11, and U12 snRNAs in anti-HA immunoprecipitates from Mat67 > UASp-HHF-Miso ovaries compared to control w1118 samples, normalized to act5c, gapdh, and rp49 mRNAs. Mean ± SD (n = 3). P-value < 0.05 (Student's t-test, unpaired, two-tailed) is indicated by *.

This Article

  1. Published in Advance April 1, 2025, doi: 10.1261/rna.080311.124 RNA 31: 822-835

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