RNA-binding protein Miso/CG44249 is crucial for minor splicing during oogenesis in Drosophila

Reduced fecundity and fertility in miso mutant females. (A and B) Female fertility assays. (A) Egg counts from test females mated with wild-type (Oregon-R) males. (B) Egg hatching rates. Mean ± SD (n = 6, 7, 6, 6, and 3, for miso^+/+, miso^null/+, miso^null/null, miso^null/null + miso-EGFP, and miso^null/null + nos > Miso, respectively). P-value <0.05 (Student's t-test, unpaired, two-tailed) is indicated by *. (C) Male fertility assays showing progeny counts from test males crossed with wild-type (Oregon-R) virgin females. Mean ± SD (n = 6 for miso^+/+ and 4 for miso^null/+, miso^null/null, and miso^null/null + miso-EGFP).