RNA-binding protein Miso/CG44249 is crucial for minor splicing during oogenesis in Drosophila

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FIGURE 1.
FIGURE 1.

Domain structure, mutant allele, and expression of Miso. (A) Domain structures of Drosophila Miso (CG44249) protein, its null mutant allele generated in this study, and human RNPC3 (65K). (B) miso mRNA expression profile, obtained from FlyBase (FBgn0265184). (C) Western blot analysis of Miso protein expression. (D) Confocal images of germarium and egg chambers from miso-EGFP transgenic flies showing Miso-EGFP (green) and DAPI-stained nuclei (blue). Scale bars, 10 μm for germarium and stage 3, 5, and 7 egg chambers; 50 μm for stage 10 egg chamber. (E) Western blot of ovary lysates, with nonspecific bands marked by asterisk (*). (F) Western blot of lysates of ovaries, female carcass after ovary dissection, testes, and male carcass after testis dissection, with nonspecific bands marked by asterisk (*).

This Article

  1. Published in Advance April 1, 2025, doi: 10.1261/rna.080311.124 RNA 31: 822-835

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