Results of RNA sequencing of mRNAs in the hippocampus of Tent2 KO and WT mice. (A) Differential expression of mRNAs in Tent2 KO hippocampi versus WT (n = 3/genotype). Genes with significantly changed expression are highlighted (red, upregulated; blue, downregulated). The data were shrunken for clarity. (B) Gene ontology analysis of transcripts downregulated in Tent2 KO mice. (C) Differential adenylation analysis between Tent2 KO and WT mice. Dashed lines indicate significance cutoff values. (D) Global distribution of poly(A) tail lengths of mRNAs in Tent2 KO and WT mice (n = 3 mice/genotype). (E) Distribution of poly(A) tail lengths in mRNAs with expression downregulated in RNA-seq. (F) Distribution of poly(A) tail lengths in mRNAs with expression downregulated in RNA-seq. For panels D–F, dashed lines indicate median values (also provided above the plotting area). (G) Pearson's correlation analyses on mRNA and miRNA expression levels. Analysis included mono(A)-tailed isomiRs with predicted binding sites in genes that were significantly deregulated under Tent2 KO. Violet, inverse correlation; brown, positive correlation. A distinct gene assigned to a potential biological process that they regulate.
