Aaeg Dcr2 PAZ domain mutations result in dysregulated vsiRNA duplex overlap lengths. (A) Heat maps showing mean overlap probability z-scores of 18–30 nt SFV-derived small RNAs from AF319 transiently expressing Dcr2 PAZ domain mutant M1–M4 or WT Dcr2, or eGFP (control) from two independent repeats, as initially characterized in Figure 4. Differing lengths of vsiRNAs, analyzed individually, are shown horizontally, and nucleotide overlaps are listed vertically. The red arrow labeled Dcr2 indicates the expected 2 nt overlap from dsRNA cleavage with cells boxed in black. The black arrow labeled pp (ping-pong) shows the expected 10 nt overlap from potential ping-pong amplification in the piRNA pathway. (B) Number of overlapping pairs per million mapped reads of 19–23 nt virus-derived small RNAs from AF319 transiently expressing Dcr2 PAZ domain mutants M1–M4 or WT Dcr2, or eGFP (control) infected with SFV. Data are shown as the mean of two independent repeats with the range of values.
