Aaeg Dcr2 PAZ domain mutations affected 21 nt vsiRNA magnitude and length. (A) Small RNA sequencing of AF319 cells, transiently expressing Dcr2 PAZ domain mutants M1–M4 or WT Dcr2, or eGFP (as negative control), and infected with SFV (MOI = 1) at 48 hpi. Histogram of small RNA reads 18–30 nt in length, mapped to the SFV (SFV4 GenBank ID: KP699763) genome (positive numbers) and antigenome (negative numbers), with colors indicating first base nucleotide prevalence per size, shown as mean percentage of mapped reads (y-axis, percentage reads) from two independent experiments ±SD. Mapping of SFV-derived 21 nt vsiRNAs, mapped along the SFV genome (magenta) or antigenome (cyan) (y-axis, vsiRNA reads per million) ±SD. (B) Fold change of magnitude of SFV-derived small RNAs of lengths 20–22 nt of Dcr2 PAZ domain mutants M1–M4 compared to WT Dcr2.
