FIGURE 2.
Mutation and expression of Aaeg Dcr2. (A) Table showing produced Aaeg Dcr2 PAZ domain mutants M1–M4, the respective introduced loss-of-function mutations, as well as corresponding amino acid residues in Dm Dcr2; aa, amino acid. (B) Assessment of (myc-tagged) Aaeg Dcr2 expression by western blot analysis. AF319 cells were transfected with either pPUb plasmids expressing WT Dcr2 or Dcr2 PAZ domain mutants M1–M4, using pPUb-myc-eGFP as control. Anti-myc and anti-α-tubulin (control) antibodies were used. Representative of three independent repeats (other repeats in Supplemental Fig. S2).
