The PAZ domain of Aedes aegypti Dicer 2 is critical for accurate and high-fidelity size determination of virus-derived small interfering RNAs

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FIGURE 1.
FIGURE 1.

Domain features of Dcr2. (A) Schematic representation of D. melanogaster and Ae. aegypti Dcr2, including functional domains: helicase domain, domain of unknown function (DUF), PAZ domain, RNase IIIA and IIIB domains, and dsRNA-binding domain (dsRBD); aa, amino acid. (B) Multiple sequence alignment of insect Dcr2 referring to Aaeg Dcr2 AAW48725, including the selected mutations of highly conserved amino acids for potential PAZ domain loss of function. Accession numbers for sequences used are indicated in Supplemental Table S1. Alignment was produced using Benchling (https://www.benchling.com/).

This Article

  1. Published in Advance February 13, 2025, doi: 10.1261/rna.080149.124 RNA 31: 679-691

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