TABLE 1.
Medium composition for ES cell derivation
| Component | Ratio/concentration | Unit | Manufacturer |
|---|---|---|---|
| Neurobasal | 2 | – | Gibco |
| DMEM | 1 | – | |
| F-12 | 1 | – | |
| N2 | – | – | |
| B27 | – | – | |
| l-glutamine | 2 | mM | |
| Nonessential amino acids | 0.1 | mM | |
| BSA (bovine serum albumin) | 0.1 | % | Sigma-Aldrich |
| β-Mercaptoethanol | 0.1 | mM | |
| Penicillin | 5000 | Units/mL | Gibco |
| Streptomycin | 5000 | Units/mL | |
| LIF (leukemia inhibitory factor) | 1000 | IU/mL | Chemicon International |
| MEK1 inhibitor PD0325901 | 1 | µM | Sigma-Aldrich |
| GSK3 inhibitor CHIR99021 | 3 | µM | Tocris Bioscience |
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The components above the thick bar are media composing the final medium used, mixed in the ratio denoted in the ratio/concentration column. Components below the thick bar are additional components supplemented to the medium.
