DIS3L, cytoplasmic exosome catalytic subunit, is essential for development but not cell viability in mice

Analysis of proteins interacting with DIS3L in mice. (A) Distribution of proteins identified by mass spectrometry analysis of DIS3L-GFP co-precipitated peptides from Dis3l^GFP/GFP protein liver extracts presented as peptide abundance [log₁₀(mean LFQ intensity in GFP samples/molecular weight of identified protein)] relative to peptide specificity [log₁₀(mean LFQ intensity in GFP samples/LFQ intensity in WT sample)]. (B) Blow out of specifically enriched proteins section of A marked by red rectangle. All nine mammalian core exosome proteins co-precipitated with DIS3L-GFP as the most specific and abundant proteins detected in the experiment. Also marked are all identified factors involved in translation regulation. (C) Functional enrichment analysis of proteins co-precipitated with DIS3L-GFP. Only several terms with the highest P-value are presented. Identified terms are grouped by data source subgroups: biological process (BP), molecular function (MF), and cellular component (CC). A full list of identified terms is available in Supplemental Fig. S2.