New reporters for monitoring cellular NMD

Verification of NMD defects in hits from finalized mutant screen. Quantitative PCR for the ratio of unspliced precursor of RPL28 mRNA to the mature RPL28 mRNA. TAF10 was used for normalization. Error bars depict standard deviation (n = 3). Statistically significant results using a single sample t-test are marked with (*). Each tested strain is shown on the x-axis, and the column color indicates the condition. The wt baseline value of one is depicted as an arrow.