Schizosaccharomyces pombe pus1 mutants are temperature sensitive due to decay of tRNA^Ile(UAU) by the 5′-3′ exonuclease Dhp1, primarily targeting the unspliced pre-tRNA

A schematic of two stable predicted conformations of S. pombe WT pre-tRNA^Ile(UAU) and three variants predicted to have structure compatible with splicing. Structures are shown for the complete unspliced pre-tRNA^Ile(UAU), with the UAU anticodon outlined in red, arrows indicating the 5′ and 3′ splice sites, and numbers at the bottom represent the predicted folding free energy change (kcal/mol) of the pre-tRNA. The colors of nucleotides are as in Figure 7A, corresponding to the probability of each base pair. Structures were obtained using the RNAstructure program (https://rna.urmc.rochester.edu/RNAstructureWeb/).