TABLE 6.
Mutations in the sequence on the 5′ side of the terminator stem affect the binding of the rpmG-3′ and AniS-3′ to N. meningitidis ProQ
| 32P-RNA | Kd [nM] (maximal fraction bound) |
|---|---|
| rpmG-3′ | 1 ± 0.52 (89%)a |
| rpmG-2AtoU | 1.7 ± 0.81 (91%) |
| rpmG-3AtoU | 4.2 ± 2.7 (78%) |
| AniS-3′ | 5 ± 0.4 (91%)a |
| AniS-2UtoA | 2.8 ± 0.55 (88%) |
| AniS-3UtoA | 0.3 ± 0.073 (97%) |
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The Kd values were obtained by fitting the data using the quadratic equation, except the data for AniS-3UtoA which was fit using the equation for one site-specific binding with the Hill slope model. The average Kd values with standard deviations were calculated from at least three independent experiments.
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aData from Table 1.
