TABLE 4.
The bottom part of the terminator stem of rpmG-3′ and AniS-3′ RNAs is recognized by N. meningitidis ProQ
| 32P-RNA | Kd [nM] (maximal fraction bound) |
|---|---|
| rpmG-3′ | 1 ± 0.52 (89%)a |
| rpmG-loop | 0.5 ± 0.34 (81%) |
| rpmG-loop-44 | 4 ± 2 (91%) |
| rpmG-loop-40 | 1.2 ± 1.5 (80%) |
| rpmG-loop-36 | 0.13 ± 0.14 (64%) |
| AniS-3′ | 5 ± 0.4 (91%)a |
| AniS-loop | 0.39 ± 0.18 (53%) |
| AniS-loop-39 | 1.1 ± 1.1 (72%) |
| AniS-loop-35 | 1 ± 0.31 (75%) |
| AniS-loop-31 | 8.3 ± 3.7 (82%) |
| AniS-loop-29 | 5.8 ± 3.9 (81%) |
| AniS-loop-27 | 4.4 ± 2.8 (62%) |
| AniS-25 | >50% (9%) |
| AniS-23 | n.d. |
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The Kd values were obtained by fitting the data using the quadratic equation, except for the data for the AniS-loop construct, which were fit using the equation for one site-specific binding with the Hill slope model. The average Kd values with standard deviations were calculated from at least three independent experiments. (n.d.) The binding was essentially undetected up to 50 nM concentration of ProQ.
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aData from Table 1.
