TABLE 3.
The length of the sequence on the 5′ side of the Rho-independent terminator affects the binding of rpmG-3′ and AniS-3′ RNAs to N. meningitidis ProQ
| 32P-RNA | Kd [nM] (maximal fraction bound) |
|---|---|
| rpmG-3′ | 1 ± 0.52 (89%)a |
| rpmG-45 | 2.8 ± 1.7 (90%) |
| rpmG-35 | 0.84 ± 0.23 (71%) |
| rpmG-33 | 7.3 ± 2.5 (63%) |
| rpmG-31 | >50 (8%) |
| rpmG-29 | n.d. |
| rpmG-26 | n.d. |
| AniS-3′ | 5 ± 0.4 (91%)a |
| AniS-40 | 3.9 ± 4.4 (83%) |
| AniS-37 | 3.9 ± 1.2 (60%) |
| AniS-35 | >50 (2%) |
| AniS-33 | n.d. |
| AniS-31 | n.d. |
| AniS-29 | n.d. |
-
The Kd values were obtained by fitting the data using the quadratic equation. The average Kd values with standard deviations were calculated from at least three independent experiments. (n.d.) The binding was essentially undetected up to 50 nM concentration of ProQ.
-
aData from Table 1.
