TABLE 2.
The length of the 3′ single-stranded tail following the Rho-independent terminator affects the binding of rpmG-3′ and AniS-3′ RNAs to N. meningitidis ProQ.
| 32P-RNA | Kd [nM] (maximal fraction bound) |
|---|---|
| rpmG-U6AU2 | 0.22 ± 0.26 (88%) |
| rpmG-U6AU | 0.2 ± 0.23 (89%) |
| rpmG-U6A | <0.5 (66%)a |
| rpmG-3′ | 1 ± 0.52 (89%)b |
| rpmG-U5 | 0.47 ± 0.27 (85%) |
| rpmG-U4 | 2.6 ± 3.5 (58%) |
| rpmG-U2 | >50 (27%) |
| rpmG-noU | >50 (6%) |
| AniS-3′ | 5 ± 0.4 (91%)b |
| AniS-U7 | 0.82 ± 0.21 (88%) |
| AniS-U6 | 0.7 ± 0.62 (79%) |
| AniS-U5 | 6.5 ± 1.3 (90%) |
| AniS-U4 | 0.9 ± 0.58 (53%) |
| AniS-U2 | >50 (14%) |
| AniS-noU | n.d. |
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The Kd values were obtained by fitting the data using the quadratic equation. The average Kd values with standard deviations were calculated from at least three independent experiments. (n.d.) The binding was essentially undetected up to 50 nM concentration of ProQ.
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aThe data could not be accurately fit, and the Kd range was estimated based on the data points distribution versus the protein concentration.
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bData from Table 1.
