RNA recognition by minimal ProQ from Neisseria meningitidis

TABLE 1.

Equilibrium RNA binding to N. meningitidis ProQ

32P-RNA Kd [nM] (maximal fraction bound)
AniS-3′ 5 ± 0.4 (91%)
Bns1 0.65 ± 0.54 (97%)
carA-5′ 1.3 ± 0.42 (88%)
iga-3′ 0.3 ± 0.28 (51%)
intergenic 1.4 ± 1 (86%)
pnp-5′ 7.9 ± 0.8 (63%)
rpmG-3′ 1 ± 0.52 (89%)
  • The Kd values were obtained by fitting the data using the quadratic equation. The average Kd values with standard deviations were calculated from at least three independent experiments.

This Article

  1. RNA 31: 549-565