Thermodynamic control of mismatch discrimination for extensive splicing regulation of PKM pre-mRNA

TABLE 4.

Mismatch discrimination properties of modified sSSOs

Name	Sequence (5′-3′)	$\text{[math]}$ (kcal/mol) sE10	$\text{[math]}$ (kcal/mol) sI9	$\text{[math]}$ (kcal/mol)	$\text{[math]}$ (kcal/mol)
sSSO	CGGAGUCA	12.01	5.46	−6.55	0.00
sSSO1	C^LGGAGUCA	13.12	5.73	−7.39	−0.84
sSSO2	CGGA^LGUCA	13.42	5.37	−8.05	−1.50
sSSO3	CGGAGUC^LA	13.00	6.02	−6.98	−0.43
sSSO4	CGGAGUCA^L	13.23	6.03	−7.20	−0.65
sSSO5	C^UGGAGUCA	10.78	5.37	−5.41	1.14
sSSO6	CGGA^UGUCA	6.45	5.46	−0.99	5.56
sSSO7	CGGAGUC^UA	8.24	5.86	−2.38	4.17
sSSO8	CGGAGUCA^U	10.74	5.22	−5.52	1.03