TABLE 3.

Thermodynamic parameters of UNA-modified sSSO/RNA duplexes

Duplex type	SSO sequence (5′-3′)	Complementary RNA fragment sequence (5′-3′)	$\text{[math]}$ vs. log C_T
Duplex type	SSO sequence (5′-3′)	Complementary RNA fragment sequence (5′-3′)	−ΔH° (kcal/mol)	−ΔS° (eu)	$\text{[math]}$ (kcal/mol)	T_M (°C)	$\text{[math]}$ (kcal/mol)	ΔT_M (˚C)
sSSO/sE10	CGGAGUCA (sSSO)	UGACUCCG	68.5 ± 1.2	182.3 ± 3.5	12.01 ± 0.08	64.0	0.00	0.00
	C^UGGAGUCA (sSSO5)	UGACUCCG	61.6 ± 2.4	163.8 ± 7.3	10.78 ± 0.15	60.0	1.23	−4.00
	CGGA^UGUCA (sSSO6)	UGACUCCG	55.0 ± 2.0	156.5 ± 6.5	6.45 ± 0.04	36.5	5.56	−27.50
	CGGAGUC^UA (sSSO7)	UGACUCCG	59.9 ± 6.9	166.4 ± 22.0	8.24 ± 0.22	46.1	3.77	−17.90
	CGGAGUCA^U (sSSO8)	UGACUCCG	63.8 ± 2.3	171.2 ± 7.1	10.74 ± 0.12	58.9	1.27	−5.10
sSSO/sI9	CGGAGUCA (sSSO)	GUACACCA	49.8 ± 4.7	143.1 ± 16.5	5.46 ± 0.22	30.5	0.00	0.00
	C^UGGAGUCA (sSSO5)	GUACACCA	45.3 ± 1.5	128.7 ± 5.2	5.37 ± 0.07	29.2	0.09	−1.30
	CGGA^UGUCA (sSSO6)	GUACACCA	45.0 ± 5.8	127.5 ± 19.5	5.46 ± 0.35	29.8	0.00	−0.70
	CGGAGUC^UA (sSSO7)	GUACACCA	41.4 ± 3.2	114.4 ± 10.7	5.86 ± 0.16	32.0	−0.4	1.50
	CGGAGUCA^U (sSSO8)	GUACACCA	50.9 ± 6.4	147.3 ± 21.37	5.22 ± 0.35	29.2	0.24	−1.30

Thermodynamic control of mismatch discrimination for extensive splicing regulation of PKM pre-mRNA