TABLE 2.

Thermodynamic parameters of LNA-modified sSSO/RNA duplexes

Duplex type	SSO sequence (5′-3′)	Complementary RNA fragment sequence (5′-3′)	$\text{[math]}$ vs. log C_T
Duplex type	SSO sequence (5′-3′)	Complementary RNA fragment sequence (5′-3′)	−ΔH° (kcal/mol)	−ΔS° (eu)	$\text{[math]}$ (kcal/mol)	T_M (°C)	$\text{[math]}$ (kcal/mol)	ΔT_M (°C)
sSSO/sE10	CGGAGUCA (sSSO)	UGACUCCG	68.5 ± 1.2	182.3 ± 3.5	12.01 ± 0.08	64.0	0.00	0.00
	C^LGGAGUCA (sSSO1)	UGACUCCG	73.5 ± 1.5	194.5 ± 4.3	13.12 ± 0.12	67.6	−1.11	3.6
	CGGA^LGUCA (sSSO2)	UGACUCCG	74.0 ± 8.6	195.4 ± 25.1	13.42 ± 0.81	68.8	−1.41	4.8
	CGGAGUC^LA (sSSO3)	UGACUCCG	69.7 ± 4.2	182.7 ± 12.7	13.00 ± 0.32	68.7	−0.99	4.7
	CGGAGUCA^L (sSSO4)	UGACUCCG	75.7 ± 5.1	201.3 ± 15.1	13.25 ± 0.38	67.2	−1.24	3.2
sSSO/sI9	CGGAGUCA (sSSO)	GUACACCA	49.8 ± 4.7	143.1 ± 16.5	5.46 ± 0.22	30.5	0.00	0.00
	C^LGGAGUCA (sSSO1)	GUACACCA	40.7 ± 4.1	112.8 ± 13.6	5.73 ± 0.20	31.0	−0.27	0.5
	CGGA^LGUCA (sSSO2)	GUACACCA	48.9 ± 3.4	140.3 ± 11.4	5.37 ± 0.16	29.8	0.09	−0.7
	CGGAGUC^LA (sSSO3)	GUACACCA	43.2 ± 2.0	119.9 ± 6.8	6.02 ± 0.08	33.4	−0.56	2.9
	CGGAGUCA^L (sSSO4)	GUACACCA	38.1 ± 4.3	103.4 ± 14.4	6.03 ± 0.27	33.0	−0.57	2.5

Thermodynamic control of mismatch discrimination for extensive splicing regulation of PKM pre-mRNA