Thermodynamic control of mismatch discrimination for extensive splicing regulation of PKM pre-mRNA

The normalized expression of PKM1 (A) and PKM2 (B) mRNA in response to transfection of SSOs with single or multiple UNA or LNA modifications. The expression of PKM1 and PKM2 mRNA was normalized against untreated cells mRNA. The error bars represent the standard deviation for six biological repeats. The statistical significance, marked with * and ***, was calculated with one-way ANOVA test. (*) P < 0.05; (***) P < 0.001.