DRBD18 acts as a transcript-specific RNA editing auxiliary factor in Trypanosoma brucei

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FIGURE 4.
FIGURE 4.

DRBD18 is associated with a subset of mitochondrial transcripts. (Top) Western blot showing degree of DRBD18 enrichment in three biological replicate α-DRBD18 IPs; empty beads served as the control (Con). In, input. Eight mitochondrial transcripts were quantified in DRBD18 IPs using qRT-PCR primers that amplify the total mRNA population. Values shown are fold enrichment in the DRBD18 pulldown compared to the empty bead control. The experiment was performed in three biological replicates, each with three technical qRT-PCR triplicates, and significance was calculated by Student's t-test. (ns) Not significant; (*) P < 0.05; (**) P < 0.01, (***) P < 0.001.

This Article

  1. RNA 31: 245-257