Full gene PCR analysis of A6 and COIII mRNA editing upon DRBD18 depletion and overexpression. (A) Agarose gel analysis of A6 and COIII RT-PCR reactions using RNA isolated from DRBD18 RNAi cells that were grown in the absence or presence of doxycycline (Doxy) for 20 h. Reverse transcription was carried out with an oligo(dT) primer that primes on the poly(A) tails of mitochondrial RNAs. Subsequent PCR was done with primers specific to the 5′ and 3′ ends of the A6 and COIII mRNAs to amplify the entire population of mRNAs including preedited, partially edited, and fully edited. (B) Agarose gel analysis of A6 and COIII RT-PCR reactions using RNAs isolated from DRBD18 overexpression cells that were grown in the absence or presence of doxycycline for 36 h. The reaction was performed as described in A.
