Effect of DRBD18 depletion and overexpression on U-indel RNA editing. (A, top) Western blot showing degree of DRBD18 knockdown in three biological replicate DRBD18 RNAi samples. Knockdown levels are quantitated on the right. (Bottom) qRT-PCR analysis of RNA isolated from uninduced (−Doxy) and induced (+Doxy) DRBD18 RNAi cells using primer sets designed to detect total, preedited, and edited mRNA of a subset of mitochondrial transcripts. The relative abundance of each transcript in induced versus uninduced RNAi cells is shown, and the RNA levels were normalized to 18S rRNA. Three biological replicates, each with three technical replicates, were performed. (B, top) Western blot showing degree of DRBD18 overexpression in three biological replicates. Levels of total DRBD18 (endogenous plus 2XMyc-tagged DRBD18) are shown on the right. (Bottom) qRT-PCR analysis of RNA isolated from uninduced (−Doxy) and induced (+Doxy) DRBD18 overexpression cells using the same sets of primers as described in A.
