Rational design yields RNA-binding zinc finger domains with altered sequence specificity

TABLE 4.

RNA-binding profile in RBNS of all double mutants

ZnF double mutant Mutant RNA-binding profile
N22 N32 Affinity Predominant kpLogo
R R Similar GGU
R H Lower GGG
D R Lower GGG
D H N/A N/A
E R Lower GGG
E D N/A N/A
E E N/A N/A
E Q N/A N/A
E H N/A N/A
E S N/A N/A
Q R Lower GGG
Q H N/A N/A
H R Similar GGG
H H Lower GGG
S R Lower GGG
S D N/A N/A
S E N/A N/A
S Q N/A N/A
S H N/A N/A
S S N/A N/A

This Article

  1. Published in Advance December 10, 2024, doi: 10.1261/rna.080329.124 RNA 31: 150-163

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