TABLE 2.
RNA-binding profile in RBNS of all single mutants
| Single mutant | Mutant RNA-binding profile | ||
|---|---|---|---|
| Affinity | Top 10 sequence motifs | Predominant kpLogo | |
| R27D | N/A | N/A | N/A |
| R27E | N/A | N/A | N/A |
| R27N | Lower | AUGGGU | GGG |
| GUGGGU | |||
| AUGGUA | |||
| GGUGGG | |||
| GUGGUA | |||
| GUGGGG | |||
| AUGGGA | |||
| UUGGGU | |||
| ACGGGA | |||
| AUGGGG | |||
| R27Q | Lower | AUGGGU | GGG |
| AUGGUA | |||
| GUGGGU | |||
| GUGGUA | |||
| ACGGUA | |||
| GGUGGG | |||
| AUGGGA | |||
| ACGGGA | |||
| GUAGGU | |||
| GUGGGG | |||
| R27H | Lower | GUGGGU | GGU |
| GUGGUA | |||
| UGGGUG | |||
| GUGGUU | |||
| UGGGUA | |||
| GUAGGU | |||
| UUGGUA | |||
| AUGGUA | |||
| GGUGGU | |||
| UUGGGU | |||
| R27S | Lower | UGGGUA | GGU |
| GUGGGU | |||
| ACGGGU | |||
| AUGGUA | |||
| AUGGGU | |||
| ACGGUA | |||
| UGGGUG | |||
| GUGGGG | |||
| GUAGGU | |||
| UUGGUA | |||
| R28D | N/A | N/A | N/A |
| R28E | N/A | N/A | N/A |
| R28N | N/A | N/A | N/A |
| R28Q | N/A | N/A | N/A |
| R28H | N/A | N/A | N/A |
| R28S | N/A | N/A | N/A |
| N32R | Similar | UGGGUA | GGG |
| UUGGUA | |||
| AUGGUA | |||
| UGGGGU | |||
| ACGGGU | |||
| GUGGGU | |||
| UGGGUU | |||
| UUGGUG | |||
| GUGGUA | |||
| UUGGGU | |||
| N32D | N/A | N/A | N/A |
| N32E | N/A | N/A | N/A |
| N32Q | Lower | AUGGGU | GGG |
| UUGGUA | |||
| AUGGUA | |||
| GUGGGU | |||
| UGGGUA | |||
| GGUGGG | |||
| UUGGGU | |||
| GUGGUA | |||
| AGGGGA | |||
| UGGGUG | |||
| N32H | Lower | UUGGUA | GGU |
| ACGGGU | |||
| UGGGUA | |||
| AUGGUA | |||
| AUGGGU | |||
| UAGGUA | |||
| UGGGGU | |||
| GUGGGU | |||
| GUGGUA | |||
| UUGGGU | |||
| N32S | N/A | N/A | N/A |
