Rational design yields RNA-binding zinc finger domains with altered sequence specificity

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FIGURE 3.
FIGURE 3.

RBNS results of selective ZnF single mutants. (A–E) Top 10 enriched RNA 6-mers of R27N, R27Q, N32Q, R27H, and R27S. This group of single mutants showed overall lower RNA-binding affinity than the wild-type ZnF, despite retaining some degree of specificity. (F) KpLogo results of R27N, R27Q, N32Q, R27H, and R27S. (G) Top 10 enriched RNA 6-mers and kpLogo results of N32H. (H) Top 10 enriched RNA 6-mers and kpLogo results of N32R, which retained RNA-binding specificity and RNA-binding affinity.

This Article

  1. Published in Advance December 10, 2024, doi: 10.1261/rna.080329.124 RNA 31: 150-163

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